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dc.contributor.authorБайляк, Марія Михайлівна-
dc.contributor.authorБурдилюк, Надія Ігорівна-
dc.contributor.authorBayliak, Maria-
dc.contributor.authorBurdylyuk, Nadia-
dc.date.accessioned2020-11-20T08:54:34Z-
dc.date.available2020-11-20T08:54:34Z-
dc.date.issued2017-
dc.identifier.citationBayliak M., Burdyliuk N. Effects of long-term cultivation on medium with alpha-ketoglutarate supplementation on metabolic processes of Saccharomyces cerevisiae. Journal of Aging Research. 2017. Vol. 2017, article ID 8754879, 12 pagesuk_UA
dc.identifier.urihttp://hdl.handle.net/123456789/8689-
dc.description.abstractDuring last years, alpha-ketoglutarate (AKG), an important intermediate in the Krebs cycle, has been intensively studied as a dietary supplement with stress-protective and potential antiaging effects. Here, we examined the effects of exogenous AKG on metabolic processes and survival of yeast Saccharomyces cerevisiae during long-term cultivation. Growth on AKG had no effect on the total cell number but increased the number o freproductively active cells at the late days of cultivation (from day7today15).A gradual increase in levels of total protein, glycogen, and trehalose was found over 7-day cultivation with more pronounced effects in AKGgrown cells. In control cells, metabolic activity and the activities of superoxide dismutase and catalase decreased, whereas levels of carbonyl proteins and low-molecular-mass thiols increased during 7-day cultivation. This suggests development of oxidative stress in stationary phase cells. Meanwhile, stationary phase cells cultured on AKG possessed higher levels of low-molecular-mass thiols and lower levels of carbonyl proteins and𝛼-dicarbonyl compounds when compared to control ones. Collectively, higher levels of storage carbohydrates and an activation of antioxidant defense with diminishing oxidative protein damage can prevent a loss of reproductive ability in yeast cells during long-term cultivation on AKG-supplemented medium.uk_UA
dc.language.isoenuk_UA
dc.publisherHindawiuk_UA
dc.relation.ispartofseries;8754879-
dc.subjectyeast Saccharomyces cerevisiae, antioxidant defense, alpha-ketoglutarate, oxidative stress, metabolismuk_UA
dc.titleEffects of Long-Term Cultivation on Medium with Alpha-Ketoglutarate Supplementation on Metabolic Processes of Saccharomyces cerevisiaeuk_UA
dc.typeArticleuk_UA
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