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Title: | Effects of Long-Term Cultivation on Medium with Alpha-Ketoglutarate Supplementation on Metabolic Processes of Saccharomyces cerevisiae |
Authors: | Байляк, Марія Михайлівна Бурдилюк, Надія Ігорівна Bayliak, Maria Burdylyuk, Nadia |
Keywords: | yeast Saccharomyces cerevisiae, antioxidant defense, alpha-ketoglutarate, oxidative stress, metabolism |
Issue Date: | 2017 |
Publisher: | Hindawi |
Citation: | Bayliak M., Burdyliuk N. Effects of long-term cultivation on medium with alpha-ketoglutarate supplementation on metabolic processes of Saccharomyces cerevisiae. Journal of Aging Research. 2017. Vol. 2017, article ID 8754879, 12 pages |
Series/Report no.: | ;8754879 |
Abstract: | During last years, alpha-ketoglutarate (AKG), an important intermediate in the Krebs cycle, has been intensively studied as a dietary supplement with stress-protective and potential antiaging effects. Here, we examined the effects of exogenous AKG on metabolic processes and survival of yeast Saccharomyces cerevisiae during long-term cultivation. Growth on AKG had no effect on the total cell number but increased the number o freproductively active cells at the late days of cultivation (from day7today15).A gradual increase in levels of total protein, glycogen, and trehalose was found over 7-day cultivation with more pronounced effects in AKGgrown cells. In control cells, metabolic activity and the activities of superoxide dismutase and catalase decreased, whereas levels of carbonyl proteins and low-molecular-mass thiols increased during 7-day cultivation. This suggests development of oxidative stress in stationary phase cells. Meanwhile, stationary phase cells cultured on AKG possessed higher levels of low-molecular-mass thiols and lower levels of carbonyl proteins and𝛼-dicarbonyl compounds when compared to control ones. Collectively, higher levels of storage carbohydrates and an activation of antioxidant defense with diminishing oxidative protein damage can prevent a loss of reproductive ability in yeast cells during long-term cultivation on AKG-supplemented medium. |
URI: | http://hdl.handle.net/123456789/8689 |
Appears in Collections: | Наукові видання (ФПН) |
Files in This Item:
File | Description | Size | Format | |
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burdyliuk&bayliak2017.pdf | Наукова стаття | 1.86 MB | Adobe PDF | View/Open |
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